In vitro method has been developed for propagation ofWrightia tinctoria R.Br. using cotyledonary node segments. Murashige and Skoog's (MS) medium supplemented with 5.0 mg dm−3 of 6-benzylaminopurine (BAP) and 0.01 mg dm−3 of naphthaleneacetic acid (NAA) induced up to eight shoots per explant with an average shoot length of 1.4 cm in 21 d. Three fold multiplication rate was achieved during every subculture of regenerated shoots on the same medium producing an average of 230 shoots per node within 84 d. Reduction in BAP concentration from 5.0 to 1.0 mg dm−3 during subculture promoted shoot length without affecting the rate of multiplication. The differentiated shoots could be rooted by a dip treatment into preautoclaved indole-3-butyric acid (IBA-500 mg dm−3 for 5 min) followed by their implantation onto MS medium containing 1/4 salts. Rooting was observed within 8–10 d in approximately 80% of shoots inoculated after IBA treatment. 15 d after rooting, the plantlets were transferred to culture bottles containing soil-SoilriteTM (1∶1) and liquid nutrient solution comprising 1/4 MS salts. After their partial hardening in these bottles for 10 d they were transferred to pots containing soil-Soilrite (1∶1) mixture with 60% transplantation success. Methods are being standardized to improve the rate of survival and large scale field transfer.