In order to obtain insight into the basic mechanism of cytoplasmic undulation in early spermatids, tubuli seminiferi of young adult rats were fixed in Stieve’s solution and examined immunohistochemically with anti-fast skeletal muscle myosin serum. A number of nuclei of spermatogonia, spermatocytes and spermatids stained intensely, but in addition the cytoplasm of early spermatids showed a positive reaction. It is suggested that myosin is involved in the cytoplasmic movements of earlier spermatids. The findings indicate a connection between contractile proteins and sterility in man.