Introduction ..... ... ...... ....... .. .. ... 587 Early Developments in Plant Embryo Culture 588 Applications of Embryo Culture ......... 589 Recent Developments in Embryo Culture 591 M ed ia .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 591 Use of Plant Extracts 598 Amino Acids 600 Carbohydrates 601 Use of Agar 603 Effects of Auxins 604 pH of Media 606 Temperature and Light 607 Gases 607 Experimental Embryology 608 Culture of Bryophyte Embryos ......... 608 Culture of Fern Embryos 608 Culture of Gymnosperm Embryos 609 Culture of Angiosperm Embryos ......... 610 Conclusion 614 Literature Cited 616