Positioning of screw holes is an important production procedure for steel construction connecting with bolts. In this paper, a new production method is presented, in which the digital camera is used for taking pictures of screw holes and other techniques are advanced. This paper also indicates that the pixels of CCD chip in photogrammetry should be chosen as all geometric units in an image, such as interior elements and all kinds of distortions. The measure can also simplify the camera calibration for determining the size of non-square pixel.