A procedure is described to regenerate shoots and plants from the embryonic axis of avocado seedlings with benzyladenine or thidiazuron. Explants were grown in the dark for 10 d and then transferred to an 18-h photoperiod to induce multiple shoots. Increased concentrations of benzyladenine or thidiazuron resulted in increased shoot production. Shoots were transferred to shoot promoting media where additional shoots were formed. Under the best conditions, six rooted shoots were produced per original explant. About 80% of the shoots with roots survived in the glasshouse and produced normal phenotypic plants.