Amniotic fluid total proteins and its fractions were estimated in 45 samples at different gestations. An increase in protein concentration was observed from 14 to 28 weeks of gestation followed by a fall. The fall of values beyond 28 weeks was statistically significant with a narrow scatter around the mean. Beyond 14 weeks of gestation protein value of 0.26 gm/dl or less probably indicates a mature fetus with gestation of 37 weeks or more. Study of protein fractions (polyacrylamide gel electrophoresis method) was not helpful in the assessment of fetal maturity.