Irradiation and fusion gene transfer

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1995 English Publisher:Springer Science and Business Media LLCJournal:Molecular Biotechnology, volume 3, pages 117-128 (issn: 1073-6085, eissn: 1559-0305,

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Authors: M A, Walter; P N, Goodfellow;

doi: 10.1007/bf02789107

pmid: 7620972

Irradiation and fusion gene transfer

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Abstract

Irradiation and fusion gene transfer (IFGT) is a technique that spans the gap between the limitations of molecular methods and somatic-cell genetics, allowing the separation of DNA fragments between 0.25 and 30 Mb in size. In conjunction with genetic linkage analysis and physical mapping techniques, IFGT provides a very useful addition to methods for cloning disease loci, and mapping chromosomes and entire genomes.

Related Organizations

University of Alberta
Canada
University of Cambridge
United Kingdom

Keywords

Genetic Markers, Recombination, Genetic, Chromosome Mapping, Hybrid Cells, Chromosomes, Cell Line, Cell Fusion, Mice, Radiation Chimera, Animals, Chromosomes, Human, Humans

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