Enzymatic synthesis of flavin adenine dinucleotide
Copyright policy )Enzymatic synthesis of flavin adenine dinucleotide
A new and simple enzymatic method for the synthesis of flavin adenine dinucleotide (FAD) from flavin mononucleotide by the transadenylylation reaction using microbial cells is described. Among various microorganisms tested, Artherobacter globiformis IFO 12138 and two soil bacteria were selected as useful enzyme sources. Under suitable reaction conditions, the amount of FAD synthesized was 2.25 mumol/mL with cells of A. globiformis. The transadenylylation reaction could be coupled with the ATP supplying system through a glycolysis process with yeast.
- Kyoto University Japan
Bacteria, Species Specificity, Flavin-Adenine Dinucleotide, Indicators and Reagents, Arthrobacter, Nucleotidyltransferases, Soil Microbiology
Bacteria, Species Specificity, Flavin-Adenine Dinucleotide, Indicators and Reagents, Arthrobacter, Nucleotidyltransferases, Soil Microbiology
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