Thermal cycle dideoxy DNA sequencing
Copyright policy )Thermal cycle dideoxy DNA sequencing
Thermal cycle dideoxy DNA sequencing eliminates the requirements for independent primer annealing and double-stranded DNA denaturation steps. The method enables sequencing from nanogram amounts of DNA from double-stranded and single-stranded PCR products, and plasmid or phage DNA templates. Thermal cycle sequencing also enables direct sequencing from bacterial colonies or phage plaques. Protocols using the Vent exo- DNA polymerase, helpful suggestions, and a troubleshooting guide are also presented.
- New England Biolabs (United Kingdom) United Kingdom
- New England Biolabs United States
Hot Temperature, Temperature, DNA, Single-Stranded, Nucleic Acid Hybridization, Sequence Analysis, DNA, Templates, Genetic, Nucleic Acid Denaturation, Polymerase Chain Reaction, Dideoxynucleosides, Adenosine Triphosphate, Autoradiography, DNA Primers
Hot Temperature, Temperature, DNA, Single-Stranded, Nucleic Acid Hybridization, Sequence Analysis, DNA, Templates, Genetic, Nucleic Acid Denaturation, Polymerase Chain Reaction, Dideoxynucleosides, Adenosine Triphosphate, Autoradiography, DNA Primers
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