A trypsin inhibitor from amaranth (Amaranthus cruentus) leaves

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 2000 English Publisher:Springer Science and Business Media LLCJournal:Applied Biochemistry and Microbiology, volume 36, pages 466-468 (issn: 0003-6838, eissn: 1608-3024,

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Authors: E. V. Levleva; Yu. A. Rudenskaya; A. V. Zimacheva; V. V. Mosolov;

doi: 10.1007/bf02731890

A trypsin inhibitor from amaranth (Amaranthus cruentus) leaves

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Abstract

A protein that inhibited the proteolytic activity of trypsin was isolated from amaranth leaves (Amaranthus cruentus) by affinity chromatography on trypsin-Sepharose. The inhibition was noncompetitive (withp-nitroanilide-N-α-benzoyl-DL-arginine as substrate) and had aK i, of 1.87 × 10−7 M. The protein caused a weaker inhibitory effect on chymotrypsin, had no effect on subtilisin, displayed a molecular weight of 8 kDa, and contained no cysteine residues.

Related Organizations

Russian Academy of Sciences
Russian Federation
Lomonosov Moscow State University
Russian Federation

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