
doi: 10.1007/bf02716953
Azospirillum brasilense, an associative diazotrophs from sorghum roots grows autotrophically on NH 4 + and CaCO3. NH 4 + a is also oxidized to NO 2 - and then denitrified. Addition of malate to the autotrophic medium enhances both NH 4 + oxidation as well as NONH 2 - dissimilation. The incomplete nitrification linked denitrification results in a rapid loss of nitrogen from the growth medium. The bacterium also shows assimilatory NO 3 - and NO 2 - reductases and fixes nitrogen at 50 μg N/ml of NH 4 + NO 3 - or NO 2 -
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