Abstract(+) Gossypol, 2,2′‐bi[8‐formyl‐1,6,7‐trihydroxy‐5‐isopropyl‐3‐methylnaphthyl], has been isolated as the (+) dianilino derivative from cottonseed grown in widely different areas of the cottonbelt of the United States. The optically active derivative obtained had rotations varying from 43% to 54.7% of the value reported for the optically pure enantiomer. Attempts to resolve racemic gossypol by chemical means were not successful, but (+) gossypol was obtained by solvent fractionation (7.2%) and by chromatography on β‐lactose (2.2%).