A new budding yeast has been isolated from soil. Its most striking feature is the formation, generally preceded by isogamous or heterogamous conjugation, of unusually large multispored asci. The organism possesses a strong fermentative ability, fermenting glucose, galactose, saccharose and raffinose for one-third. Nitrate is not assimilated. It forms a pellicle in malt extract. A pseudomycelium is also formed. Cytological examination showed the vegetative cells to be uninucleate. The relationship which this yeast shows with theDipodascaceae and, in particular, withDipodascus uninucleatus Biggs, is discussed. For the classification of the yeast the new genusKluyveromyces was created. For the species the nameKluyveromyces polysporus is proposed. The Latin diagnosis of both the genus and the species is given.