AbstractDifferential scanning calorimetry of whole teliospores and lipid extracts ofTilletia controversa Kühn andT. tritica Tul. indicated that the lipid composition of teliospores was different than earlier reported. An exothermic peak at −40 to −45°C and an endotherm at −25 to −15°C indicated that the majority of lipids were triacylglycerols (TAG). Hot isopropanol was used to inactivate lipases during lipid extraction. Thin‐layer chromatographic analysis of extracted lipids showed that free fatty acids (FFA) were not present in great quantities unless water was present during lipid extraction. As measured by gas chromatography. FFA accounted for 1–5% of the lipid content in teliospores ofTilletia spp. The TAG content of teliospores was 60–80% of total lipids.