AbstractA group of unusual triglycerides, in which one of the acyl groups is a vicinal dihydroxy acid with one of the hydroxyl groups acetylated, has been isolated fromCardamine impatiens L. (Cruciferae) seed oil. Hydrolysis of these triglycerides with castor bean lipase facilitated isolation and identification of a mixture of C18, C20, C22, and C24 hydroxy acetoxy fatty acids. Pancreatic lipase hydrolysis data revealed that these monoacetylated dihydroxy acid residues are esterified exclusively with one of the α‐positions of the glycerol moiety. The remaining acyl groups are comprised of ordinary C18 unsaturated acids (which occupy 98% of the β‐position), palmitic acid, and C20, C22, and C24 monoenoic fatty acids.