Orientation of myelin proteolipid protein in the oligodendrocyte cell membrane
Copyright policy )Orientation of myelin proteolipid protein in the oligodendrocyte cell membrane
The orientation of proteins within a cell membrane can often be difficult to determine. A number of models have been proposed for the orientation of the myelin protein, proteolipid protein (PLP), each of which includes exposed domains on the intracellular and extracellular membrane faces. Immunolabeling experiments have localized the C-terminus and the region spanning amino acids 103-116 to the cytoplasmic face of the membrane, but no well characterized antibodies have been available that label extracellular PLP domains. In this report, we describe the generation and characterization of mouse monoclonal antibodies (mAb) against putative extramembrane domains. Three of the mAb, specific for PLP peptides 40-59, 178-191, or 215-232, immunostain live oligodendrocytes, indicating that these regions of the molecule are exposed on the external surface of the cell. In addition, we have used these mAb to study the time-course of incorporation of PLP into the oligodendrocyte membrane. These studies increase our knowledge of the orientation of PLP in the lipid bilayer and are relevant for understanding myelin function.
- University of Queensland Australia
- University of Queensland Australia
Identification, 1303 Biochemistry, Bovine Brain Myelin, Molecular Sequence Data, 2804 Cellular and Molecular Neuroscience, oligodendrocytes, Expression, 612, Mice, membrane orientation, proteolipid protein, Nervous-System Myelin, Antibody Specificity, antibodies, Encephalitogenic Determinant, Animals, Amino Acid Sequence, Myelin Proteolipid Protein, Cells, Cultured, Brain Chemistry, Mice, Inbred BALB C, Cell Membrane, Sjl Mice, Antibodies, Monoclonal, Immunohistochemistry, Peptide Fragments, myelin, Basic-Protein, Oligodendroglia, Animals, Newborn, Female, extracellular domains, Arrangement, Organization
Identification, 1303 Biochemistry, Bovine Brain Myelin, Molecular Sequence Data, 2804 Cellular and Molecular Neuroscience, oligodendrocytes, Expression, 612, Mice, membrane orientation, proteolipid protein, Nervous-System Myelin, Antibody Specificity, antibodies, Encephalitogenic Determinant, Animals, Amino Acid Sequence, Myelin Proteolipid Protein, Cells, Cultured, Brain Chemistry, Mice, Inbred BALB C, Cell Membrane, Sjl Mice, Antibodies, Monoclonal, Immunohistochemistry, Peptide Fragments, myelin, Basic-Protein, Oligodendroglia, Animals, Newborn, Female, extracellular domains, Arrangement, Organization
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