Vital staining of the corneal endothelium with Rose Bengal and Alizarin Red S

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 1977 English Publisher:Springer Science and Business Media LLCJournal:Albrecht von Graefe's Archive for Clinical and Experimental Ophthalmology, volume 201, pages 257-261 (issn: 0065-6100, eissn: 1435-702X,

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Authors: G A, Peyman; D J, Spence;

doi: 10.1007/bf02385183

pmid: 66883

Vital staining of the corneal endothelium with Rose Bengal and Alizarin Red S

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Abstract

A new technique for the staining of the corneal endothelium is presented, in which the actions of the vital stain, rose bengal, and the intercellular stain, alizarin red S, are combined. The technique is an improvement over other simple evaluative methods, because it defines both healthy and damaged cells, and thereby permits an investigator to accurately quantify endothelial cell damage.

Related Organizations

University of Illinois System
United States
University of Illinois at Urbana Champaign
United States

Keywords

Cornea, Tissue Survival, Rose Bengal, Staining and Labeling, Animals, Anthraquinones, Endothelium, Rabbits, Arylsulfonates

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