The application of high-voltage pulses to biological tissue causes not only electroporation, a non-thermal phenomenon of pore creation within a lipid membrane due to an elevated electric field, but also significant heating. Once a biological membrane is porated, the current density increases several times, causing Joule heating. A combined experimental and theoretical study is reported. The theoretical temperature rise for a 1.25 kV cm(-1), 6 ms pulse is about 11.2 K for a tissue conductivity of 0.5 S m(-1) (i.e. myocardial tissue) during high-voltage application. Owing to the inhomogeneous electric field obtained with the use of needle electrodes, the temperature rises first at the electrodes, where the field strength reaches a maximum. Only for highly conductive tissue such as muscle was a temperature effect primarily observed in the bulk. Even if the temperature effect is biologically insignificant, it can affect the creation of stabile aqueous pathways by electroporation. The calculation of temperature distribution during high-voltage application, taking the electric field strength and the heat transfer into account, can be a useful tool for electrode optimisation.