Production of antibodies to amanitins as the basis for their radioimmunoassay

descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1975 English Publisher:Springer Science and Business Media LLCJournal:Experientia, volume 31, pages 1,233-1,234 (issn: 0014-4754, eissn: 1420-9071,

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Authors: L, Fiume; C, Busi; G, Campadelli-Fiume; C, Franceschi;

doi: 10.1007/bf02326810

pmid: 1204754

Production of antibodies to amanitins as the basis for their radioimmunoassay

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Abstract

The production of antibodies against amanitins is described. By means of these antibodies, a radioimmunoassay was developed which allows detection of as little as 0.5 ng of amanitins in 1 ml of serum. By this method, the clearance of alpha-amanitin from the blood of poisoned mice was measured.

Related Organizations

Alma Mater Studiorum University of Bologna
Italy

Keywords

Male, Mice, Amanitins, Freund's Adjuvant, Radioimmunoassay, Animals, Rabbits, Serum Albumin, Rats

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