The linkage relationship of 11 bean (Phaseolus vulgaris) seed proteins (including phaseolin), 9 enzyme loci, and theP locus were analyzed in backcross and F2 progenies by use of the software package "Mapmaker." The progenies were obtained by crossing the breeding line 'XR-235-1' and the cultivar 'Calima'. Allelic differences for seed protein loci were detected with SDS-PAGE and those for enzyme loci with starch gel electrophoresis and activity stains. The seed coat color of 'Calima' is a red/beige mottled pattern and that of 'XR-235-1' is white. Segregation at theP locus was followed by recording the phenotype of the BC1S1 and F3 seed. A linkage group comprising ca. 90 cM was detected with the following gene order:Est-2 - 11 -Pha - 8 - (Spe/Spg) - 24 - P - 9 - (Spa/Spv) - 16 -Spba - 22 -Mdh-1. In addition, another linkage group was detected: (Spd/Spf/Sph) - 5 -Spca. Therefore, the seed proteins appear to be organized in clusters in the bean genome.