[35S] methionine-labelled Varicella-Zoster-Virus (VZV) was partly purified by sucrose density gradient centrifugation. Enveloped virus, checked by electron microscopy, was analyzed by high resolution polyacrylamide gel electrophoresis. A polypeptide pattern of at least 31 proteins could be identified on the fluorogram. The molecular weight of the proteins, estimated by co-electrophoresis with marker proteins, ranged from 18,000 to 240,000 daltons. Immunoprecipitation using a specific human antiserum showed at least 14 proteins.