According to the program “Palynological Italian Flora, Aeropalynological section”, the pollen morphological card ofPinus pinea L. is presented. The study is carried out on pollen coming from three Italian localities and regards “fresh” and acetolyzed pollen. For each sample, measurements are carried out on 30 “fresh” pollen grains in glicerol jelly with fuchsin and on 30 acetolyzed pollen grains in water/glicerol (1/1); general observations regard 1000 “fresh” and 1000 acetolyzed pollen grains/sample. Some observations on the main differences between “fresh” and acetolyzed pollen are mentioned.