Colletotrichum gloeosporioides could grow and sporulate on a wide range of pH (viz., from 3.0 to 8.5). The best growth was obtained at pH 6.0. Mannitol proved to be the best carbon source. Good growth and sporulation were also observed on maltose, glucose, galactose and sucrose. Nitrates supported better growth than ammonium compounds. Glutamic acid was found to be the best amino acid. Nitrites inhibited the growth completely at acid pH values but they supported growth at alkaline pH. Mannitol-glutamic acid was most suitable carbon-nitrogen combination for growth. Magnesium sulphate was the only sulphur source which was good both for growth and sporulation. The organism could not grow on media lacking carbon, nitrogen or sulphur.