Trypanosoma cruzi: metabolic labeling of trypomastigote sialoglycolipids

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1985 English Publisher:Springer Science and Business Media LLCJournal:Experientia, volume 41, pages 736-738 (issn: 0014-4754, eissn: 1420-9071,

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Authors: A S, Couto; B, Zingales; R M, de Lederkremer; W, Colli;

doi: 10.1007/bf02012572

pmid: 3891398

Trypanosoma cruzi: metabolic labeling of trypomastigote sialoglycolipids

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Abstract

Trypomastigote forms (infective) of Trypanosoma cruzi incorporate (3H)-palmitic acid and D-(3H)-galactose into glycolipids. Palmitic acid-labeled acidic glycolipids were partially hydrolyzed with neuraminidase. The labeling of these compounds when the intact cell surface was labeled with galactose oxidase plus NaB3H4 indicates the membrane location of the sialoglycolipids.

Related Organizations

University of Buenos Aires
Argentina
Universidade de São Paulo
Brazil
Facultad de Ciencias Agrarias y Forestales
Argentina
UNIVERSIDADE DE SAO PAULO
Brazil

Keywords

Trypanosoma cruzi, Cell Membrane, Palmitic Acid, Galactose, Borohydrides, Palmitic Acids, Tritium, Galactose Oxidase, Kinetics, Animals, Glycolipids

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