Specific determination of arylsulfatase A activity

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1986 English Publisher:Springer Science and Business Media LLCJournal:Experientia, volume 42, pages 33-35 (issn: 0014-4754, eissn: 1420-9071,

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Authors: H, Inoue; Y, Seyama; S, Yamashita;

doi: 10.1007/bf01975881

pmid: 2867927

Specific determination of arylsulfatase A activity

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Abstract

Arylsulfatase activities in biological materials are too low to be detected by the methods available hitherto. A sensitive and specific assay method for arylsulfatase A (AS-A) has been developed in the present study. Ascorbate-2-sulfate is known to be a specific natural substrate of AS-A; the ascorbic acid liberated by the action of AS-A was quantitatively determined using HPLC equipped with an amperometric detector. The method was used to analyze the activity of AS-A in biological materials.

Keywords

Leukocytes, Animals, Brain, Humans, Ascorbic Acid, Sulfatases, Cerebroside-Sulfatase, Chromatography, High Pressure Liquid, Rats

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