Enzyme immunoassay for arginine vasopressin

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 1982 English Publisher:Springer Science and Business Media LLCJournal:Experientia, volume 38, pages 786-787 (issn: 0014-4754, eissn: 1420-9071,

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Authors: T, Uno; K, Uehara; K, Motomatsu; E, Ishikawa; K, Kato;

doi: 10.1007/bf01972270

pmid: 6809485

Enzyme immunoassay for arginine vasopressin

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Abstract

A highly sensitive enzyme immunoassay for arginine vasopressin (AVP) was described. The enzyme used was β-D-galactosidase fromEscherichia coli, and it was coupled to AVP by using the N-hydroxysuccinimide ester of N-(4-carboxycyclohexylmethyl)-maleimide. The complex was then utilized for the enzyme immunoassay, which could detect 0.5 fmoles (or 0.5 pg) of AVP in the assay tube.

Related Organizations

Miyazaki Welfare Medical College
Japan

Keywords

Arginine Vasopressin, Immunoenzyme Techniques, Maleimides, Escherichia coli, Humans, Lypressin, Oxytocin, beta-Galactosidase

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