Muscle fluorometry: A determination of the depth of penetration

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1985 English Publisher:Springer Science and Business Media LLCJournal:Experientia, volume 41, pages 456-458 (issn: 0014-4754, eissn: 1420-9071,

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Authors: A W, Stewart;

doi: 10.1007/bf01966144

pmid: 3987867

Muscle fluorometry: A determination of the depth of penetration

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Abstract

Fluorometric recordings of NADH (nicotinamide adenine dinucleotide) were made on rabbit papillary muscles. The specimens were placed between the UV light source and a small window of the detection stage. As the muscle was moved over the window in a transverse direction, simultaneous measurements could be taken of transmitted UV light and fluorescent light for various thicknesses of tissue. It is concluded that a muscle thickness of 0.65 mm is optimal before absorption of the incident light decreases the fluorescence signal.

Related Organizations

Monash University, Clayton campus
Australia
Monash University
Australia

Keywords

Muscles, Animals, Scattering, Radiation, Fluorometry, Rabbits, Papillary Muscles, NAD, Fluorescence, Mathematics

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