Detergent solubilization of cardiac 5′-nucleotidase

descriptionPublicationkeyboard_double_arrow_right Article 01 May 1983 English Publisher:Springer Science and Business Media LLCJournal:Experientia, volume 39, pages 507-508 (issn: 0014-4754, eissn: 1420-9071,

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Authors: G, Lutoslawska; N, Jha; H P, Baer;

doi: 10.1007/bf01965178

pmid: 6303829

Detergent solubilization of cardiac 5′-nucleotidase

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Abstract

Nonionic, anionic and zwitterionic detergents were employed for solubilization of 5′-nucleotidase from acetone powder preparations of rat heart. Zwittergents effectively solubilized the enzyme and may allow now its further identification and purification by electrophoretic techniques.

Related Organizations

University of Alberta
Canada

Keywords

Myocardium, Detergents, Polidocanol, Polyethylene Glycols, Rats, Betaine, Quaternary Ammonium Compounds, Surface-Active Agents, Solubility, Nucleotidases, Animals, 5'-Nucleotidase, Deoxycholic Acid

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