Affinity chromatography of glucose dehydrogenase
Copyright policy )Affinity chromatography of glucose dehydrogenase
Porcine liver beta-D-glucose dehydrogenase, a multi-functional protein, has been purified to apparent homogeneity. The enzyme has been separated from the endoplasmic reticulum using Triton X-114 and further purified using NAD to release glucose dehydrogenase from a NADP-linked sepharose column. The purified enzyme is capable of producing both NADH and NADPH in vivo as indicated by kinetic studies.
- Wichita State University United States
Electrophoresis, 570, Swine, Glucose Dehydrogenases, 610, Research Support, Endoplasmic Reticulum, P.H.S, Chromatography, Affinity, Glucose Dehydrogenases/isolation & purification, Animals, Non-U.S. Gov't, Chromatography, Polyacrylamide Gel, Endoplasmic Reticulum/enzymology, Liver/enzymology, Glucose 1-Dehydrogenase, NADP/metabolism, NAD, Glucose Dehydrogenases/metabolism, Kinetics, Liver, Carbohydrate Dehydrogenases/isolation & purification, Carbohydrate Dehydrogenases, Electrophoresis, Polyacrylamide Gel, U.S. Gov't, NAD/metabolism, Isoelectric Focusing, NADP
Electrophoresis, 570, Swine, Glucose Dehydrogenases, 610, Research Support, Endoplasmic Reticulum, P.H.S, Chromatography, Affinity, Glucose Dehydrogenases/isolation & purification, Animals, Non-U.S. Gov't, Chromatography, Polyacrylamide Gel, Endoplasmic Reticulum/enzymology, Liver/enzymology, Glucose 1-Dehydrogenase, NADP/metabolism, NAD, Glucose Dehydrogenases/metabolism, Kinetics, Liver, Carbohydrate Dehydrogenases/isolation & purification, Carbohydrate Dehydrogenases, Electrophoresis, Polyacrylamide Gel, U.S. Gov't, NAD/metabolism, Isoelectric Focusing, NADP
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