S-adenosyl-L-homocysteine: A non-cytotoxic hypomethylating agent
Copyright policy )S-adenosyl-L-homocysteine: A non-cytotoxic hypomethylating agent
The cytotoxic effect caused by the hypomethylating agent S-adenosyl-L- homocysteine (SAH) was compared with that of two drugs commonly used to induce DNA hypomethylation, 5-azacytidine and 5-aza-2'-deoxycytidine. Two in vitro cytotoxicity tests, the tetrazolium MTT assay and the intracellular lactate dehydrogenase (LDH) activity test, suggest that SAH induces hypomethylation without causing any cytotoxic effect. We propose the use of SAH as a non-cytotoxic agent which may be more suitable for inducing experimental DNA hypomethylation.
DNA Replication, Cell Membrane Permeability, L-Lactate Dehydrogenase, Tetrazolium Salts, DNA, Fibroblasts, Decitabine, Methylation, S-Adenosylhomocysteine, Mitochondria, Azacitidine, Humans, DNA Modification Methylases, Cells, Cultured
DNA Replication, Cell Membrane Permeability, L-Lactate Dehydrogenase, Tetrazolium Salts, DNA, Fibroblasts, Decitabine, Methylation, S-Adenosylhomocysteine, Mitochondria, Azacitidine, Humans, DNA Modification Methylases, Cells, Cultured
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).25 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!