On the solvent-extraction of acetylcholine receptor

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1978 English Publisher:Springer Science and Business Media LLCJournal:Experientia, volume 34, pages 689-691 (issn: 0014-4754, eissn: 1420-9071,

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Authors: J P, Christopher; D B, Millar; M A, Grafius;

doi: 10.1007/bf01947259

pmid: 658271

On the solvent-extraction of acetylcholine receptor

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Abstract

Chloroform/methanol rechromatography on Sephadex LH-20 of fractions isolated from organic solvent extracts of E. electricus electric organs exhibited alterations in elution point compared to the original chromatography. Additionally, 3H decamethonium bromide did not coelute with any of the observed fractions. It is concluded that as presently described the organic solvent isolation procedure for the cholinergic receptor exhibits undesirable characteristics.

Related Organizations

Walter Reed National Military Medical Center
United States
United States Department of the Navy
United States
Naval Medical Research Center
United States

Keywords

Electric Organ, Methanol, Electrophorus, Chromatography, Gel, Solvents, Animals, Receptors, Cholinergic, Chloroform, Acetylcholine

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