An improved test for Africanized honeybee mitochondrial DNA

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1991 English Publisher:Springer Science and Business Media LLCJournal:Experientia, volume 47, pages 968-969 (issn: 0014-4754, eissn: 1420-9071,

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Authors: Y C, Crozier; S, Koulianos; R H, Crozier;

doi: 10.1007/bf01929894

pmid: 1915781

An improved test for Africanized honeybee mitochondrial DNA

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Abstract

Mitochondrial DNA derived from Apis mellifera scutellata, the ancestor of the Africanized bees of the New World, lacks a BglII restriction site found in other types of honeybee. We present primers allowing amplification of a 485-bp section of the cytochrome b gene containing this site, using the polymerase chain reaction. Digestion of the amplified product with BglII yields contrasting patterns between Africanized and other honeybees.

Related Organizations

La Trobe University
Australia

Keywords

Base Sequence, Africa, Molecular Sequence Data, Restriction Mapping, Animals, Bees, Cytochrome b Group, DNA, Mitochondrial

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