Expression of the Tn antigen on erythroid cells from a patient with Tn syndrome
Copyright policy )Expression of the Tn antigen on erythroid cells from a patient with Tn syndrome
In order to examine expression of the Tn antigen on erythroid cells from a patient with Tn syndrome, we applied a selective two phase liquid culture system for human erythroid progenitors in peripheral blood. The cells were analyzed with flow cytometry employing an anti-Tn antibody and a lectin of Vicia villosa which recognizes only the Tn determinant. In the second phase, the Tn antigen was expressed on the cultured cells from the patient on day 3 and Tn-positive cells reached 62.7% on day 9. On the other hand, Tn-positive cells were not detected in the volunteer's cultured cells. When the patient's cells were co-cultured with the cells from a healthy volunteer, the percentage of Tn-positive cells was much lower than the expected value, suggesting that the normal cells suppressed the expression of Tn antigen on the patient's cells.
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Erythrocyte Aggregation, Erythroid Precursor Cells, Male, Acetylgalactosamine, Anemia, Refractory, with Excess of Blasts, Membrane Glycoproteins, Erythrocytes, Abnormal, Cell Differentiation, Hemagglutination Tests, Syndrome, Middle Aged, Flow Cytometry, Galactosyltransferases, Hematologic Diseases, Antigens, Surface, Humans, Antigens, Tumor-Associated, Carbohydrate
Erythrocyte Aggregation, Erythroid Precursor Cells, Male, Acetylgalactosamine, Anemia, Refractory, with Excess of Blasts, Membrane Glycoproteins, Erythrocytes, Abnormal, Cell Differentiation, Hemagglutination Tests, Syndrome, Middle Aged, Flow Cytometry, Galactosyltransferases, Hematologic Diseases, Antigens, Surface, Humans, Antigens, Tumor-Associated, Carbohydrate
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