HLA-DP typing by amplified fragment length polymorphisms (AFLPs)

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1990 English Publisher:Springer Science and Business Media LLCJournal:Immunogenetics, volume 32, pages 56-59 (issn: 0093-7711, eissn: 1432-1211,

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Authors: J W, Dekker; S, Easteal;

doi: 10.1007/bf01787330

pmid: 2373527

HLA-DP typing by amplified fragment length polymorphisms (AFLPs)

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Abstract

Here we present an alternative PCR-based method of HLA-DP typing that involves analysis of electrophoretically separated restriction fragments, or amplified fragment length polymorphisms (AFLPs), produced by digestion of PCR-amplified DPB exons 2 sequences at allele-specific restriction sites.

Related Organizations

Australian National University
Australia

Keywords

HLA-DP Antigens, Polymorphism, Genetic, Base Sequence, Molecular Sequence Data, Restriction Mapping, Humans, Electrophoresis, Polyacrylamide Gel, Oligonucleotide Probes, Polymerase Chain Reaction, Alleles

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