Protection of rabbits against HTLV-II infection with a synthetic peptide corresponding to HTLV-II neutralization region
Copyright policy )Protection of rabbits against HTLV-II infection with a synthetic peptide corresponding to HTLV-II neutralization region
Rabbit immune sera raised against synthetic peptides of the HTLV-II envelope gp46 region were examined for HTLV-II neutralization ability by HTLV-vesicular stomatitis virus (VSV) pseudotype assay and syncytium inhibition assay. HTLV-II neutralization activity was detected in the sera against HTLV-II Env gp46, 80-103 but not in those to HTLV-II Env gp46, 171-196. Three rabbits immunized with the synthetic peptide of HTLV-II Env gp46, 80-103 and three non-immunized rabbits were challenged with intravenous inoculation of an HTLV-II-producing human cell line (MOT, 1 x 10(7) cells). The non-immunized rabbits showed seroconversion for HTLV-II after 2 weeks and maintained persistent infection but the immunized rabbits were protected from HTLV-II infection. Nested or repeated polymerase chain reaction revealed the presence of HTLV-II provirus sequences in the non-immunized rabbits but not in the immunized rabbits. These results suggest that peptide vaccination with a synthetic peptide corresponding to the HTLV-II neutralization region is useful for preventing HTLV-II infection.
- Okayama University Japan
- Gunma University Japan
- Kitasato University Japan
Vaccines, Synthetic, Base Sequence, Sequence Homology, Amino Acid, Deltaretrovirus Antibodies, Human T-lymphotropic virus 2, Molecular Sequence Data, Retroviridae Proteins, Oncogenic, Gene Products, env, Viral Vaccines, Giant Cells, Cell Line, Disease Models, Animal, Neutralization Tests, DNA, Viral, HTLV-II Infections, Animals, Humans, Amino Acid Sequence, Rabbits, DNA Primers
Vaccines, Synthetic, Base Sequence, Sequence Homology, Amino Acid, Deltaretrovirus Antibodies, Human T-lymphotropic virus 2, Molecular Sequence Data, Retroviridae Proteins, Oncogenic, Gene Products, env, Viral Vaccines, Giant Cells, Cell Line, Disease Models, Animal, Neutralization Tests, DNA, Viral, HTLV-II Infections, Animals, Humans, Amino Acid Sequence, Rabbits, DNA Primers
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