A conserved sequence motif at the 5′ terminus of the Southampton virus genome is characteristic of the Caliciviridae

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1995 United Kingdom English Publisher:Springer Science and Business Media LLCJournal:Virus Genes, volume 10, pages 149-152 (issn: 0920-8569, eissn: 1572-994X,

Copyright policy )

Authors: Lambden, P.R.; Liu, B.L.; Clarke, I.N.;

doi: 10.1007/bf01702595

pmid: 8560774

A conserved sequence motif at the 5′ terminus of the Southampton virus genome is characteristic of the Caliciviridae

- Summary
- Subjects
- Metrics

Abstract

We have determined the 5'terminal cDNA sequence for the genome of Southampton virus, a recently characterized, human, small round-structured virus (SRSV). Genomic RNA was extracted directly from a stool sample and amplified by RT-PCR by homopolymer tailing of the 3' terminus of the cDNA. The additional sequence increases the overall length of the Southampton virus genome by 12 nucleotides, resulting in a significant change to the genome organization by extending the first large open reading frame (ORF) by 51 amino acids. The 5'terminal bases pGpT and the presence of conserved genome and putative subgenomic RNA terminal motifs are now prominent features shared between the human SRSV Southampton virus and the animal caliciviruses rabbit hemorrhagic disease virus and feline calicivirus.

Country

United Kingdom

Related Organizations

University of Southampton
United Kingdom
Southampton General Hospital
United Kingdom
University Hospital Southampton NHS Foundation Trust
United Kingdom

Keywords

Base Sequence, Molecular Sequence Data, Genome, Viral, 630, Gastroenteritis, Norwalk virus, DNA, Viral, Humans, Amino Acid Sequence, Conserved Sequence, Caliciviridae Infections

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	39
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%