Isolation and properties of nonhistone chromosomal proteins from pea chromatin

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1973 English Publisher:Springer Science and Business Media LLCJournal:Molecular and Cellular Biochemistry, volume 1, pages 197-207 (issn: 0300-8177, eissn: 1573-4919,

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Authors: P P, Lin; R F, Wilson; J, Bonner;

doi: 10.1007/bf01659330

pmid: 4753927

Isolation and properties of nonhistone chromosomal proteins from pea chromatin

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Abstract

We have separated the nonhistone chromosomal proteins of pea chromatin from other chromosomal constituents and have studied some of the biological functions of these proteins. After dissociation of pea chromatin in 3m NaCl, 5m urea (pH 8) and subsequent removal of urea, the chromosomal proteins are separated from DNA on Bio-Gel A50. Histones are then separated from nonhistones by chromatography on Bio-Rex 70 in the presence of 0.35m NaCl and 5m urea. The resulting nonhistone proteins are concentrated on a DEAE-cellulose column.

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California Institute of Technology
United States

Keywords

Transcription, Genetic, Osmolar Concentration, Sodium Dodecyl Sulfate, DNA, Templates, Genetic, Sodium Chloride, Chromatography, Ion Exchange, Electrophoresis, Disc, Tritium, Chromatin, Chromatography, DEAE-Cellulose, Chromosomes, Histones, Molecular Weight, Centrifugation, Density Gradient, Chromatography, Gel, Chemical Precipitation, Amino Acids, Plant Proteins, Protein Binding

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