Tn1721-induced mutation in an isoprenol degrading plasmid fromPseudomonas putida

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 1986 English Publisher:Oxford University Press (OUP)Journal:Journal of Industrial Microbiology, volume 1, pages 271-274 (issn: 0169-4146, eissn: 1476-5535,

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Authors: Peter A. Vandenbergh; Ronald L. Cole;

doi: 10.1007/bf01569282

Tn1721-induced mutation in an isoprenol degrading plasmid fromPseudomonas putida

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Abstract

Transformation experiments using pRO1825 containing the tetracycline resistance transposonTn1721 were conducted to induce mutations in plasmid pSRQ50, which coded for acyclic isoprenol degradation. Transformants that were tetracycline resistant were examined for their ability to utilize geraniol. Strains unable to utilize geraniol were observed to have a 0.6 kb addition on the C fragment of pSRQ50.

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