Assignment of the gene encoding cytosolic phosphoenolpyruvate carboxykinase (GTP) toMus musculus chromosome 2

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 1985 English Publisher:Springer Science and Business Media LLCJournal:Somatic Cell and Molecular Genetics, volume 11, pages 633-638 (issn: 0740-7750, eissn: 1572-9931,

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Authors: J, Lem; R E, Fournier;

doi: 10.1007/bf01534728

pmid: 3865385

Assignment of the gene encoding cytosolic phosphoenolpyruvate carboxykinase (GTP) toMus musculus chromosome 2

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Abstract

The structural gene Pck-1, encoding cytosolic phosphoenolpyruvate carboxykinase (GTP) (PEPCK; EC 4.1.1.32), has been assigned to mouse chromosome 2. This assignment was made based on genomic Southern transfer of rat-mouse and hamster-mouse hybrid DNAs using a rat kidney cloned cDNA of the PEPCK gene as a probe. Conclusive evidence for the cosegregation of the PEPCK gene with mouse chromosome 2 was obtained using a monochromosomal microcell hybrid that selectively retained a Robertsonian translocation between mouse autosomes 2 and 8 and its back-selected hybrid clone.

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University of California System
United States
University of Southern California
United States

Keywords

Chromosome Mapping, Nucleic Acid Hybridization, Hybrid Cells, Chromosomes, Cell Line, Clone Cells, Mice, Cricetulus, Cytosol, Genes, Cricetinae, Protein Biosynthesis, Animals, Phosphoenolpyruvate Carboxykinase (GTP)

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