A technique for quantitative cytology of nasal secretions

descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1991 English Publisher:Springer Science and Business Media LLCJournal:European Archives of Oto-rhino-laryngology, volume 248, pages 406-408 (issn: 0937-4477, eissn: 1434-4726,

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Authors: H S, Lee; Y, Majima; Y, Sakakura; B W, Kim;

doi: 10.1007/bf01463564

pmid: 1747249

A technique for quantitative cytology of nasal secretions

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Abstract

To quantitatively analyze cellular elements in mucopurulent nasal secretions from patients with chronic paranasal sinusitis, we studied five mucus-liquefying agents--dithiothreitol (DTT), ethylenediaminetetraacetic acid tetrasodium salt, propylene glycol, serratio peptidase and urea--with Hanks' balanced salt solution used as a control. Agents were compared for the number of cells, the mucus-liquefying effect and the staining characteristic of cells in cytocentrifuge-prepared smears. The results indicated that the treatment of nasal secretions with 10 mM DTT provides more clear and detailed cytological preparations for quantitative cytology of nasal secretions.

Related Organizations

Catholic University of America
United States
Mie University
Japan

Keywords

Cytological Techniques, Cell Count, Propylene Glycol, Dithiothreitol, Mucus, Nasal Mucosa, Propylene Glycols, Chronic Disease, Humans, Urea, Sinusitis, Edetic Acid, Peptide Hydrolases

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