Electron microscopy of endothelial cells in culture: I. Transmission electron microscopy

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 1986 English Publisher:Springer Science and Business Media LLCJournal:Journal of Tissue Culture Methods, volume 10, pages 31-33 (issn: 0271-8057, eissn: 1573-0603,

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Authors: U. S. Ryan; M. A. Hart;

doi: 10.1007/bf01404587

Electron microscopy of endothelial cells in culture: I. Transmission electron microscopy

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Abstract

Endothelial cells in culture can be identified by structural and functional characteristics. To examine the ultrastructure of the undisturbed endothelial cell monolayer, methods are described for in situ fixation and embedment in the tissue culture flask. Subsequent sectioning and staining for electron microscopy are carried out without detachment of the cell layer from the flask.

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