Purification and characterization of equine infectious anemia virus
Copyright policy )Purification and characterization of equine infectious anemia virus
EIA virus was purified from equine fetal kidney cell cultures by PEG-precipitation, two sucrose-gradient sedimentations (5-30 per cent) and (25 to 60 per cent) centrifugation, using the immunodiffusion test to follow the procedure. Purified EIA virus had a density (20 degrees C) of 1.162 and a sedimentation constant of S20w=656. electron microscopy revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. The dense core may be at various locations inside the membrane bound particle.
- Cornell University United States
Cell Membrane, Kidney, Inclusion Bodies, Viral, Culture Techniques, Centrifugation, Density Gradient, Leukocytes, Animals, Chemical Precipitation, Horses, Infectious Anemia Virus, Equine
Cell Membrane, Kidney, Inclusion Bodies, Viral, Culture Techniques, Centrifugation, Density Gradient, Leukocytes, Animals, Chemical Precipitation, Horses, Infectious Anemia Virus, Equine
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