In vitro translation of mRNA species from cells infected with Machupo virus

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1987 English Publisher:Springer Science and Business Media LLCJournal:Archives of Virology, volume 92, pages 315-319 (issn: 0304-8608, eissn: 1432-8798,

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Authors: I S, Lukashevich; T A, Stelmakh; E P, Stchesljenok; T V, Shkolina;

doi: 10.1007/bf01317488

pmid: 3028336

In vitro translation of mRNA species from cells infected with Machupo virus

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Abstract

RNA from Machupo virus infected cells was centrifuged in a linear sucrose gradient and RNAs from gradient fractions were tested separately for template activity in a cell-free protein synthesizing system from rabbit reticulocytes. Fraction 15-16 S programmed the synthesis of protein that migrated in SDS-polyacrylamide gel like the nucleocapsid protein of a purified virus. The synthesis of virus glycoproteins was not detected in the system.

Keywords

Viral Core Proteins, Viral Proteins, Capsid, Protein Biosynthesis, Animals, RNA, Viral, RNA, Messenger, Arenaviridae, Vero Cells, Arenaviruses, New World

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