Cell culture studies with a cytopathic bovine rotavirus
Copyright policy )Cell culture studies with a cytopathic bovine rotavirus
The growth characteristics in MDBK cells of a calf rotavirus isolated in Northern Ireland are described. Of a range of cell cultures tested, the virus grew in secondary bovine kidney and MDBK cells, but consistently produced a CPE only in MDBK cells. The CPE consisted of cytoplasmic vacuolation, development of eosinophilic intracytoplasmic inclusions, and degeneration and detachment of cells from the monolayer. The onset of CPE was more rapid and its effects more severe in rolled cultures than stationary cultures. Immunofluorescent staining showed that the rotavirus was antigenically unrelated to avian and mammalian reoviruses. There was no significant difference in virus growth over the pH range 6.5 to 8.4.
Diarrhea Viruses, Bovine Viral, Haplorhini, Virus Replication, Cell Line, Epitopes, Cytopathogenic Effect, Viral, Cricetinae, Animals, RNA Viruses, Cattle, Antigens, Viral
Diarrhea Viruses, Bovine Viral, Haplorhini, Virus Replication, Cell Line, Epitopes, Cytopathogenic Effect, Viral, Cricetinae, Animals, RNA Viruses, Cattle, Antigens, Viral
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