Pulsed tunable lasers in cytofluorometry
Copyright policy )Pulsed tunable lasers in cytofluorometry
A tunable dye laser which generates pulses of high intensity (107 W cm−2) and short duration (2 nsec) was used in an experimental set-up for cytofluorometry. The fluorescence signal given by a single cell (frog erythrocytes stained by a conventional Feulgen reaction for the demonstration of DNA) has been studied. This system allows, for the first time, the fluorescence decay time to be measured directly in the cell. The system has the following advantages compared to the conventional methods: (i) absence of appreciable photodecomposition; and (ii) a potential increase in sensitivity by several orders of magnitude.
- University of Pavia Italy
- Instituto Politécnico Nacional Mexico
- National Research Council Italy
Cell Nucleus, Erythrocytes, Time Factors, Staining and Labeling, Nitrogen, Lasers, Cytological Techniques, Rana esculenta, DNA, In Vitro Techniques, Fluorescence, Animals, Fluorometry
Cell Nucleus, Erythrocytes, Time Factors, Staining and Labeling, Nitrogen, Lasers, Cytological Techniques, Rana esculenta, DNA, In Vitro Techniques, Fluorescence, Animals, Fluorometry
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