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pmid: 1456888
Antigens in a particulate conformation were shown to be highly immunogenic in mammals. For this reason, the particle forming capacity of derivatives of the HIV-1 group specific core antigen p55 gag was assayed and compared dependent on various expression systems: recombinant bacteria, vaccinia- and baculoviruses were established encoding the entire core protein p55 either in its authentic sequence or lacking the myristylation consensus signal. Moreover, p55 gag was expressed in combination with the protease (p55-PR) or with the entire polymerase (p55-pol), respectively. Budding of 100-160 nm p55 core particles, resembling immature HIV-virions, was observed in the eucaryotic expression systems only. In comparison to the vaccinia virus driven expression of p55 in mammalian cells, considerably higher yields of particulate core antigen were obtained by infection of Spodoptera frugiperda (Sf9) insect cells with the recombinant Autographa californica nuclear polyhedrosis (AcMNPV) baculovirus. Mutation of the NH2-terminal myristylation signal sequence prevented budding of the immature core particles. Expression of the HIV p55-PR gene construct by recombinant baculovirus resulted in complete processing of the p55 gag precursor molecule in this system. The introduction of an artificial frameshift near the natural frameshift site resulted in constitutive expression of the viral protease and complete processing of p55, both in Escherichia coli and in vaccinia virus infected cells. Interestingly, significant processing of p55 resembling that of HIV infected H9 cells could also be achieved in the vaccinia system by fusing the entire pol gene to the gag gene. Moreover, processing was not found to be dependent on amino-terminal myristylation of the gag procursor molecule, which is in contrast to observations with type C and type D retrovirus. However, complete processing of p55 into p24, p17, p9 and p6 abolished particle formation. Purified immature HIV-virus like particles were highly immunogenic in rabbits, leading to a strong humoral immune response after immunization. Empty immature p55 gag particles represent a noninfectious and attractive candidate for a basic vaccine component.
Oligodeoxyribonucleotides/chemistry, HIV-1/genetics, Genes, Viral, Genetic Vectors, Molecular Sequence Data, Viral Structural Proteins/genetics, 610 Medizin, Gene Products, gag, Vaccinia virus, Baculoviridae/genetics, Capsid, Escherichia coli, Protein Precursors, Cloning, Molecular, Gene Products, gag/metabolism, Recombinant Proteins/immunology, AIDS Vaccines, Viral Structural Proteins, Vaccines, Synthetic, Vaccines, Synthetic/immunology, Base Sequence, Vaccinia virus/genetics, Capsid/immunology, Protein Precursors/metabolism, Genes, gag, Recombinant Proteins, Escherichia coli/genetics, Oligodeoxyribonucleotides, HIV-1, AIDS Vaccines/immunology, Baculoviridae, Protein Processing, Post-Translational
Oligodeoxyribonucleotides/chemistry, HIV-1/genetics, Genes, Viral, Genetic Vectors, Molecular Sequence Data, Viral Structural Proteins/genetics, 610 Medizin, Gene Products, gag, Vaccinia virus, Baculoviridae/genetics, Capsid, Escherichia coli, Protein Precursors, Cloning, Molecular, Gene Products, gag/metabolism, Recombinant Proteins/immunology, AIDS Vaccines, Viral Structural Proteins, Vaccines, Synthetic, Vaccines, Synthetic/immunology, Base Sequence, Vaccinia virus/genetics, Capsid/immunology, Protein Precursors/metabolism, Genes, gag, Recombinant Proteins, Escherichia coli/genetics, Oligodeoxyribonucleotides, HIV-1, AIDS Vaccines/immunology, Baculoviridae, Protein Processing, Post-Translational
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