Development of plaque assay for vesicular exanthema of swine virus (VESV) under methyl cellulose overlay

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 1967 English Publisher:Springer Science and Business Media LLCJournal:Archiv f�r die gesamte Virusforschung, volume 21, pages 53-60 (issn: 0304-8608, eissn: 1432-8798,

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Authors: Yuan Chung Zee; S. H. Madin; A. J. Hackett;

doi: 10.1007/bf01258476

pmid: 5628589

Development of plaque assay for vesicular exanthema of swine virus (VESV) under methyl cellulose overlay

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Abstract

A plaque assay method, using 1.05% methyl cellulose overlay, was developed for vesicular exanthema of swine virus (VESV) types A48, H54, I55, and K54. The validity of this plaque method as an assay system was upheld by the well correlated dose response, and the specificity of plaque formation was demonstrated by the fact that a specific antiserum could reduce the plaque count by more than 98%. The concentration of methyl cellulose exerted an influence on the size of plaques formed by the virus, showing an inverse relationship between concentration of methyl cellulose in the overlay medium and plaque size. Adsorption of VESV to monolayers of pig kidney cells at 37° C was found to reach a maximum within 60 minutes.

Related Organizations

University of California, Berkeley
United States
Universidad Naval
Mexico

Keywords

Swine Diseases, Cytopathogenic Effect, Viral, Neutralization Tests, Swine, Culture Techniques, Methods, Animals, Picornaviridae, Methylcellulose

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