
doi: 10.1007/bf01241499
pmid: 4963726
Methods for preparation of complement fixing antigen from tissues of minks infected with mink enteritis virus have been developed. Using the CF antigen as an indicator of the presence of virus, the MEV was purified by different methods, e.g. adsorption on and elution from calcium phosphate and DEAE cellulose column chromatography. The density of MEV was found to be 1.30 to 1.33 g/cm3. Electron micrographs taken from purified and concentrated preparations showed spherical particles 250 A in diameter. The relation of mink enteritis virus and feline panleucopenia is discussed.
Immunoassay, Chromatography, Virus Cultivation, Complement Fixation Tests, Guinea Pigs, Leukopenia, In Vitro Techniques, Enteritis, Microscopy, Electron, Mink, Cats, Centrifugation, Density Gradient, Animals, Viruses, Unclassified, Female, Rabbits, Antigens, Cellulose, Chickens
Immunoassay, Chromatography, Virus Cultivation, Complement Fixation Tests, Guinea Pigs, Leukopenia, In Vitro Techniques, Enteritis, Microscopy, Electron, Mink, Cats, Centrifugation, Density Gradient, Animals, Viruses, Unclassified, Female, Rabbits, Antigens, Cellulose, Chickens
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