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image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Microchimica Actaarrow_drop_down
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Microchimica Acta
Article . 1975 . Peer-reviewed
License: Springer TDM
Data sources: Crossref
Microchimica Acta
Article . 1975
Data sources: Europe PubMed Central
https://dx.doi.org/10.1007/bf0...
Article
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A colorimetric method for the assay of lipoyl dehydrogenase

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1975 English Publisher:Springer Science and Business Media LLCJournal:Mikrochimica Acta, volume 63, pages 577-583 (issn: 0026-3672, eissn: 1436-5073, Copyright policy )
Authors: A M, Seet; K T, Lee;

doi: 10.1007/bf01217319

pmid: 1160670

A colorimetric method for the assay of lipoyl dehydrogenase

Abstract

A colorimetric method for the direct assay of lipoyl dehydrogenase is described. Enzyme reaction is stopped by ethanol precipitation. This is followed by the displacement of 1,3-bis(2′-pyridyl)-1,2-diaza-prop-2-ene (PAPHY) from a Pd(II)-PAPHY complex by reduced lipoic acid. This simple quantitative method is suitable for assay of lipoyl dehydrogenase in crude tissue extracts and in cell suspensions.

Keywords

Chemistry, Chemical Phenomena, Animals, Colorimetry, Palladium, Dihydrolipoamide Dehydrogenase

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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