Three genotypically suppressible alleles,alX4, alcA125, and niaD500, are phenotypically suppressed by aminoglycoside antibiotics. Unsuppressible alleles at these loci are unaffected as are known missense mutations at the yA and gdhA loci. This is consistent with the premise that the suppressible mutations are nonsense and that this highly-allele-specific phenotypic suppression can be used to distinguish nonsense from missense mutations of Aspergillus nidulans. Paromomycin and tobramycin are recommended for screening unknown mutations.